Rapid Identification of Legionella Pathogenicity by Surface-Enhanced Raman Spectroscopy.

Jing LI; Tian Qin; Xiao Xiao Jia; Ai Hua Deng; Xu Zhang; Wen Hui Fan; Shuai Dong Huo; Ting Yi Wen; Wen Jun Liu

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Rapid Identification of Legionella Pathogenicity by Surface-Enhanced Raman Spectroscopy.

Author: Jing LI ¹ ; Tian QIN ² ; Xiao Xiao JIA ³ ; Ai Hua DENG ⁴ ; Xu ZHANG ⁵ ; Wen Hui FAN ¹ ; Shuai Dong HUO ⁵ ; Ting Yi WEN ⁴ ; Wen Jun LIU ³ ;
Author Information

1. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
2. National Institute for Communicable Disease Control and Prevention and State Key Laboratory for Infectious Disease Prevention and Control, Chinese Centre for Disease Control and Prevention, Beijing 102206, China
3. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
4. CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
5. National Center for Nanoscience and Technology, Chinese Academy of Sciences, Beijing 100190, China.
Publication Type:Journal Article
Keywords: Gold nanoparticles; Legionella pneumophila; Pathogenicity; Surface-enhanced Raman Spectroscopy
MeSH: Cell Line; Citric Acid; chemistry; Gold; chemistry; Humans; Legionella; isolation & purification; pathogenicity; Nanoparticles; chemistry; Spectrum Analysis, Raman; methods; Time Factors; Tiopronin; chemistry; Virulence
From: Biomedical and Environmental Sciences 2015;28(6):437-444
CountryChina
Language:English
Abstract:
OBJECTIVETo establish Surface-enhanced Raman Spectroscopy (SERS) can be used as a rapid and reliable method to distinguish virulent strain and mild strain of L. pneumophila.
METHODSMortality data were collected from company departments through administrative documents, death certificates, etc. Trend analyses of cancer mortality were performed on the basis of 925 cancer deaths between 2001 and 2010.
RESULTSOur results indicated that the peaks of high virulence strains reached ⋝4000. This criterion was verified by subsequent cell experiments. In addition, we also conducted SERS rapid identification on the virulence of several collected clinical strains and obtained accurate results.
CONCLUSIONThe present study indicates that the established SERS protocol can be used as a rapid and reliable method to distinguish virulent and mildly virulent strains of L. pneumophila, which can be further used in clinical samples.