Green Tea Polyphenols Attenuate High-Fat Diet-Induced Renal Oxidative Stress through SIRT3-Dependent Deacetylation.
- Author:
Hui YANG
1
;
Xue Zhi ZUO
2
;
Chong TIAN
3
;
Dong Liang HE
1
;
Wei Jie YI
1
;
Zhuo CHEN
1
;
Pi Wei ZHANG
1
;
Shi Bin DING
4
;
Chen Jiang YING
1
Author Information
- Publication Type:Letter
- MeSH: Acetylation; drug effects; Animals; Antioxidants; pharmacology; Diet, High-Fat; adverse effects; Gene Expression Regulation, Enzymologic; drug effects; Kidney; drug effects; metabolism; Male; Oxidative Stress; drug effects; Polyphenols; pharmacology; Rats; Rats, Wistar; Reactive Oxygen Species; metabolism; Sirtuin 3; metabolism; Tea; chemistry
- From: Biomedical and Environmental Sciences 2015;28(6):455-459
- CountryChina
- Language:English
- Abstract: Fifty male Wistar rats were fed a standard chow diet or a high-fat (HF) diet, and different concentrations of green tea polyphenols (GTPs) (0.8, 1.6, and 3.2 g/L) were administered in the drinking water. We found that the malondialdehyde (MDA) level in the HF diet group was significantly higher than that in the control (CON) group (P<0.05). Decreased peroxisome proliferator-activated receptor (PPAR)-α and sirtuin 3 (SIRT3) expression, and increased manganese superoxide dismutase (MnSOD) acetylation levels were also detected in the HF diet group (P<0.05). GTP treatment upregulated SIRT3 and PPARα expression, increased the pparα mRNA level, reduced the MnSOD acetylation level, and decreased MDA production in rats fed a HF diet (P<0.05). No significant differences in total renal MnSOD and PPAR-γ coactivator-1α (PGC1-α) expression were detected. The reduced oxidative stress detected in kidney tissues after GTP treatment was partly due to the higher SIRT3 expression, which was likely mediated by PPARα.
