Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition.

Ya Rong DU; Dong Pan; Ya Xiong Chen; Gang Xue; Zhen Xin Ren; Xiao Man LI; Shi Chuan Zhang; Bu Rong HU

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Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition.

Author: Ya Rong DU ¹ ; Dong PAN ² ; Ya Xiong CHEN ¹ ; Gang XUE ² ; Zhen Xin REN ¹ ; Xiao Man LI ² ; Shi Chuan ZHANG ³ ; Bu Rong HU ¹
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1. Gansu Key laboratory of Space Radiobiology, Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu, China.
2. Gansu Key laboratory of Space Radiobiology, Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu, China
3. Department of Radiation Oncology, Sichuan Cancer Hospital, Chengdu 610041, Sichuan, China.
Publication Type:Journal Article
Keywords: Adipose-derived stem cells; High- and low-LET irradiation; Irradiation response; Three-dimensional cell culture
MeSH: Adipose Tissue; cytology; radiation effects; Cell Culture Techniques; Cell Differentiation; Flow Cytometry; Humans; Neurons; cytology; Stem Cells; cytology; radiation effects; X-Rays
From: Biomedical and Environmental Sciences 2015;28(8):549-557
CountryChina
Language:English
Abstract:
OBJECTIVEAdipose tissue distributes widely in human body. The irradiation response of the adipose cells in vivo remains to be investigated. In this study we investigated irradiation response of adipose-derived stem cells (ASCs) under three-dimensional culture condition.
METHODSASCs were isolated and cultured in low attachment dishes to form three-dimensional (3D) spheres in vitro. The neuronal differentiation potential and stem-liked characteristics was monitored by using immunofluoresence staining and flow cytometry in monolayer and 3D culture. To investigate the irradiation sensitivity of 3D sphere culture, the fraction of colony survival and micronucleus were detected in monolayer and 3D culture. Soft agar assays were performed for measuring malignant transformation for the irradiated monolayer and 3D culture.
RESULTSThe 3D cultured ASCs had higher differentiation potential and an higher stem-like cell percentage. The 3D cultures were more radioresistant after either high linear energy transfer (LET) carbon ion beam or low LET X-ray irradiation compared with the monolayer cell. The ASCs' potential of cellular transformation was lower after irradiation by soft agar assay.
CONCLUSIONThese findings suggest that adipose tissue cell are relatively genomic stable and resistant to genotoxic stress.