Downregulation of transforming growth factor-beta1 and platelet-derived growth factor gene expression by interleukin-10 in murine hepatic stellate cells in response to experimental liver injury.
- Author:
Liang-fa XIONG
1
;
Xi-sheng LENG
;
Yu-hua WEI
;
Tao LI
;
Yan-tong GUO
;
Zhi-zhong QING
;
Ji-run PENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Down-Regulation; drug effects; Genetic Therapy; Hepatocytes; drug effects; physiology; Interleukin-10; pharmacology; Liver Cirrhosis, Experimental; metabolism; pathology; therapy; Male; Platelet-Derived Growth Factor; biosynthesis; RNA, Messenger; biosynthesis; Rats; Rats, Sprague-Dawley; Stromal Cells; drug effects; physiology; Transfection; Transforming Growth Factor beta; biosynthesis; Transforming Growth Factor beta1
- From: Chinese Journal of Surgery 2005;43(5):282-286
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of interleukin-10 (IL-10) on the expression of transforming growth factor-beta(1) (TGFbeta(1)) and platelet-derived growth factor (PDGF) in hepatic stellate cells (HSC) during liver injury.
METHODSThe adenovirus vector (the titer was 1 x 10(7) efu/ml) encoded IL-10 gene was used to transfect the rat via the vein of caudal. At the same time, CCl(4) was injected into rat by a hypodermic injection. These processes went on twice a week. After eight weeks, the liver were perfused with collagenase IV and purified by density gradient centrifugation with Nycodenz for separate HSC. The level of IL-10 was measured by ELISA method; The expression of PDGF and TGFbeta(1) in HSC was detected by semi-quantitative RT-PCR and Western-blot methods.
RESULTSThe level of IL-10 in therapy group (adenovirus vector encoding IL-10 gene group) was higher than that in non-therapy group (adenovirus vector without IL-10 gene and PBS group); The expression of TGFbeta(1) mRNA, TGFbeta(1) protein and PDGF mRNA, PDGF protein in therapy group were significantly lower than that in non-therapy group (P < 0.05).
CONCLUSIONDownregulating the TGFbeta(1) and PDGF expression could be the passageway by which IL-10 alleviate the degree of proliferation and activation in hepatic stellate cells.
