Uranyl Nitrate Induced Polyuric Acute Tubular Necrosis in Rats.
- Author:
In Kyoung LIM
1
;
Kee Ho LEE
;
Byung Don HAN
;
Ja Jun JANG
;
Taik Koo YUN
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Rat Kidney; proximal tubular cells; polyuric ATN
- MeSH: Animal; Kidney Failure, Acute/chemically induced*; Kidney Function Tests; Kidney Tubular Necrosis, Acute/chemically induced*; Male; Rats; Rats, Inbred Strains; Uranium/pharmacology*; Uranyl Nitrate/pharmacology*
- From:Yonsei Medical Journal 1987;28(1):38-48
- CountryRepublic of Korea
- Language:English
- Abstract: We investigated the pathobiological course of uranyl nitrate (UN) induced polyuric acute tubular necrosis (ATN) in male Sprague Dawley rats. UN (5mg/kg 15mg/kg and 3Omg/kg) in 5% NaHCO3 induced weight loss, polydipsia, and polyuria 24 hrs after injection when compared to the controls which were treated with 5% NaHCO3 only. Twenty four hours following the injection of UN, serum creatinine and blood urea nitrogen levels had increased. These changes continued for at least 72 hours, although the concentration of uranium had decreased. Light microscopic studies conducted 24 hours after injection, revealed partial degeneration and necrosis of the proximal tubules and many casts m the distal convoluted tubules. These changes progressed for 72 hours. Despite this tubular damage, the glomeruli were relatively intact. 5 days after injection, the epithelial cells lining the proximal tubules displayed regenerative activities; these findings were more prominent after 10 days. Through electron microscopic examination, we observed the destruction of mitochondria in the proximal tubular cells, a possible cause of polyuria. Ten days post injection regenerative activities in the proximal tubular cells showed that the maturation of intracellular organelles followed the proliferation of the premature cells.
