The study on the morphology character of blood-spleen barrier.
- Author:
An-long ZHU
1
;
Hong-chi JIANG
;
Lian-xin LIU
;
Da-xun PIAO
;
Shang-ha PAN
;
Hai-quan QIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Basement Membrane; ultrastructure; Endothelial Cells; ultrastructure; Female; Macrophages; ultrastructure; Male; Rats; Rats, Wistar; Reticulocytes; ultrastructure; Spleen; blood supply; ultrastructure
- From: Chinese Journal of Surgery 2005;43(9):591-594
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the morphology and functional character of blood-spleen barrier (BSB) and establish the concept of BSB.
METHODSThirty healthy Wistar rats were studied. Ten rats were injected with 1.5 ml mixed fluid of India ink and physiological saline through the tail vein. Histological changes of the spleen in all animals were observed with light and electron microscopy, including HE, Foot, Masson staining and immunohistochemistry of CD68 and CD34.
RESULTSMost of the carbon particles were within the splenic sinuses in marginal zone but not in the white pulp after 6 h. There was a characteristic distribution of the macrophagocytes, vessel endothelial cell, reticular tissue and collagen fiber in the BSB.
CONCLUSIONSBSB, surrounding the white pulp, is composed of macrophagocytes, marginal-sinus-endothelial cells and their basement membrane, the reticular tissue (reticular cells and reticular fibers) and collagen fibers. The role of BSB is to keep the microenvironment of white pulp stable. It becomes mature while the formation of germinal center of the white pulp. The permeability of BSB changes during its development.
