Expression of melanoma antigen gene in urinary transitional cell carcinomas.
- Author:
Lin GENG
1
;
Li-zhang YU
;
Peng DU
;
Ming MA
;
Yan-qun NA
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Antigens, Neoplasm; biosynthesis; genetics; Carcinoma, Transitional Cell; genetics; metabolism; Female; Gene Expression; Humans; Male; Melanoma-Specific Antigens; Middle Aged; Neoplasm Proteins; biosynthesis; genetics; RNA, Messenger; biosynthesis; Reverse Transcriptase Polymerase Chain Reaction; Tumor Cells, Cultured; Urinary Bladder Neoplasms; genetics; metabolism
- From: Chinese Journal of Surgery 2005;43(10):667-671
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the significance of melanoma antigen (MAGE) gene expression in bladder transitional cell carcinoma (TCC).
METHODSMAGE-A1, A2, A3, A4 mRNA expression was detected by reverse transcription polymerase chain reaction (RT-PCR) in 3 clusters bladder TCC cells and 20 samples of bladder TCC patients (T(1) 7 samples, T(2) 5 samples, T(3) 6 samples, T(4) 2 samples, G(1) 1 samples, G(2) 11 samples, G(3) 8 samples). MAGE-A4 protein was detected by immunohistochemistry in 105 samples of bladder TCC patients (T(1) 35 samples, T(2) 12 samples, T(3) 26 samples, T(4) 13 samples, G(1) 13 samples, G(2) 44 samples, G(3) 48 samples).
RESULTSThree clusters bladder TCC cells had MAGE gene mRNA expression. In detection of MAGE mRNA of 20 samples of bladder TCC patients, 12 samples (60%) expressed MAGE-A1, 16 samples (80%) expressed MAGE-A2, 11 samples (55%) expressed MAGE-A3, 18 samples (90%) expressed MAGE-A4, 8 samples (40%) expressed all of MAGE-A1--A4. In 105 bladder TCC samples, 53 samples (50%) expressed MAGE-A4 protein. Strong expression (++ or +++) was significant higher in higher grade (13 samples/48 samples) or stage (14 samples/51 samples) than in lower grade (2 samples/57 samples) or stage (0 samples/35 samples).
CONCLUSIONMAGE gene highly expresses in bladder TCC. Bladder TCC of high grade or high stage has higher MAGE-A4 protein strong expression.
