Expression of albumin during hepatocyte differentiation by human bone marrow stem cells.
- Author:
Bing-lu LI
1
;
Qiang QU
;
Yu-pei ZHAO
;
Xiao-dong HE
;
Lei WANG
;
Cui-zhu CHEN
;
Zhen-yuan LIU
Author Information
- Publication Type:Journal Article
- MeSH: Albumins; biosynthesis; Bone Marrow Cells; cytology; Cell Differentiation; drug effects; Cells, Cultured; Culture Media, Conditioned; pharmacology; Fibroblast Growth Factors; pharmacology; Hepatocyte Growth Factor; pharmacology; Hepatocytes; cytology; Humans; Mesenchymal Stromal Cells; cytology; physiology
- From: Chinese Journal of Surgery 2005;43(11):713-715
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the expression pattern of albumin during the hepatocyte differentiation by human bone marrow stem cells in vitro.
METHODSHuman bone marrow cells were harvested and cultured in the presence of hepatocyte growth factor (HGF), fibroblast growth factor (FGF) and lymphocyte inhibitory factor (LIF). Cells were stained immunohistochemically by albumin specific antibody and examined under a confocal microscope. Supernatant albumin level was measured biochemically on a serial time points of the culture.
RESULTSBy this condition, the attached cells became mature morphologically in 1 week of culture. Hepatocyte-specific albumin could be detected in mature cells. The albumin level revealed a time-dependent change during a 4-week culture.
CONCLUSIONHuman bone marrow cells could be induced to differentiate to mature hepatocytes that produce and secret albumin in vitro. These cells may contribute to a stable source of hepatocytes for clinical hepatocyte transplantation and artificial liver support system.
