Effect of salidroside on salivary adenoid cystic carcinoma cells in vitro.
- Author:
Mei-hua LI
1
;
Gui-zhen ZHANG
;
Yi-shu WANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Carcinoma, Adenoid Cystic; Cell Line, Tumor; Cell Proliferation; Glucosides; Humans; Immunohistochemistry; In Vitro Techniques; Phenols; Salivary Gland Neoplasms
- From: West China Journal of Stomatology 2008;26(3):312-319
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect and mechanism of salidroside on salivary adenoid cystic carcinoma (SACC-2) cells in vitro.
METHODSTo detect the effect of salidroside on SACC-2 cells growth with CCK-8 kit, the growth curve of cells were drawn. To detect the expression of cysteine proteinase (Caspase 3 and Caspase 8) and proliferating cell nuclear antigen (PCNA) in SACC-2 cells, immunohistochemistry staining was used.
RESULTSAccording the results of CCK-8 kit detected, salidroside could inhibit the proliferation of SACC-2 cells, IC50 value was (4.99+/-0.23) microg/mL. Growing curve showed that SACC-2 cells of salidroside groups decreased with extending cell culture time. Immunohistochemistry staining showed that Caspase 3 and Caspase 8 were both strong positive expression in SACC-2 cells of salidroside groups, and poor positive expression in SACC-2 cells of control group, the difference was significant (P<0.01). The expression of PCNA was reverse (P<0.01).
CONCLUSIONSalidroside could inhibit the proliferation of SACC-2 cells and induce SACC-2 cell apoptosis in vitro, which could be a kind of antitumor medicine in the future.
