Production of interleukin-1beta, interleukin-6 and tumor necrosis factor a in cultured human fibroblast with stimulation of abstract from Actinomyces naeslundii ATCC19246.
- Author:
Li-juan ZHAO
1
;
Wen LI
;
Yan ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Actinomyces; Fibroblasts; Humans; Interleukin-1; Interleukin-1beta; Interleukin-6; Lipopolysaccharides; Teichoic Acids; Tumor Necrosis Factor-alpha
- From: West China Journal of Stomatology 2008;26(5):473-478
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the production of interleukin (IL)-1beta, IL-6 and tumor necrosis factor alpha (TNFalpha) from stimulated human fibroblast with abstract from cell wall of Actinomyces naeslundii ATCC19246.
METHODSThe abstract from the cell wall from Actinomyces naeslundii were extracted and purified with the method of purifying lipoteichoic acid(LTA) and stimulated the THP-1 with three different concentrations (1, 10, 100 mg/mL). The level of IL-1beta, IL-6 and TNFalpha in the supernatant was quantitatively analyzed by ELISA. Results Abstracts at the concentrations of 10, 100 mg/mL significantly produced IL-1beta, IL-6 and TNFalpha, especially 10 mg/mL.
CONCLUSIONThe abstract from cell wall of Actinomyces naeslundii may significantly increase IL-1beta, IL-6 and TNFalpha level in the supernatant of THP-1, and the increasing level is different with the concentrations.
