Suppression of vascular endothelial growth factor expression by vector-based small interfering RNA in human tongue squamous carcinoma cell line Tca8113.
- Author:
Da-hai YU
1
;
Ying CAO
;
Zhi-wen YAO
;
Jing LI
;
Hai-bo CHEN
;
Jie HAO
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; Cell Line; Cell Line, Tumor; Cell Proliferation; Genetic Vectors; Humans; RNA, Messenger; RNA, Small Interfering; Tongue Neoplasms; Transfection; Vascular Endothelial Growth Factor A
- From: West China Journal of Stomatology 2008;26(5):550-552
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess suppression effects of vector-based small interfering RNA (siRNA) on vascular endothelial growth factor (VEGF) expression of human tongue squamous carcinoma cell line (Tca8113) in vitro.
METHODSTwo siRNA targeting VEGF constructed in eukaryotic expression vector (Pu-VEGF-siRNA1, Pu-VEGF-siRNA2), eukaryotic expression vector as the experiment control, all of which were transfected into Tca8113 cells with Lipofectamine 2000. Non-transfection cell was used as negative control. VEGF mRNA and protein were detected by reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry and enzyme linked immunosorbent assay (ELISA), respectively.
RESULTSCompared to the experimental and negative controls, the expression of VEGF mRNA and protein were significantly decreased in the Pu-VEGF-siRNA1 group and Pu-VEGF-siRNA2 group. But there were no significant differences between two controls (P > 0.05).
CONCLUSIONVector-based siRNAs targeting VEGF are efficient in down-regulating VEGF expression in Tca8113 cells.
