Effect of estrogen on heat shock protein 70 expression in rat masseter muscle.
- Author:
Bing-yan LI
1
;
Yu ZHAO
;
Xiao-qing LI
;
Xin-zhu YI
;
Yan-min WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Estradiol; analogs & derivatives; Estrogens; Female; HSP70 Heat-Shock Proteins; Humans; Masseter Muscle; Ovariectomy; Rats; Rats, Sprague-Dawley
- From: West China Journal of Stomatology 2008;26(5):560-562
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of estrogen on heat shock protein (HSP) 70 expression in rat masseter muscle.
METHODSSixty twelve-week-old female Sprague-Dawley rats were randomly divided into three groups: Sham surgery group (control group), ovariectomy group (OVX group), ovariectomy with estradiol valerate replacement treatment group (OVX/EV group). Half of the animals were sacrificed at 4 and 8 weeks respectively, then the masseter muscle was removed. Immunohistochemistry (IHC) method was employed to study the HSP70 expression in masseter muscle.
RESULTSCompared to control group and OVX/EV group, the expression of HSP70 was significantly lower at 8 weeks in OVX group (P < 0.05). There were no significantly difference between the HSP70 expression of control group and that of OVX/EV group.
CONCLUSIONEstrogen may affect HSP70 expression in rat masseter muscle, and estrogen replacement therapy may prevent HSP70 reduction.
