Single nucleotide polymorphisms in CAPN10 gene of Chinese people and its correlation with type 2 diabetes mellitus in Han people of northern China.
- Author:
Hong-Xia SUN
1
;
Kui-Xing ZHANG
;
Wei-Nan DU
;
Jin-Xiu SHI
;
Zheng-Wen JIANG
;
Hao SUN
;
Jin ZUO
;
Wei HUANG
;
Zhu CHEN
;
Yan SHEN
;
Zhi-Jian YAO
;
Bo-Qin QIANG
;
Fu-De FANG
Author Information
- Publication Type:Journal Article
- MeSH: Calpain; genetics; Case-Control Studies; China; Diabetes Mellitus, Type 2; ethnology; genetics; Ethnic Groups; Genetic Predisposition to Disease; Humans; Polymerase Chain Reaction; Polymorphism, Single Nucleotide
- From: Biomedical and Environmental Sciences 2002;15(1):75-82
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the distribution of single nucleotide polymorphisms (SNPs) in CAPN10 gene in Chinese population and their relation with type 2 diabetes mellitus in Han people of Northern China.
METHODSCAPN10 gene was sequenced to detect SNPs in different nationalities of China. Five SNPs were chosen to perform case-control study and haplotype analysis in 156 normal Han people of Northern China and 173 type 2 diabetes. One SNP was also analyzed with transmission-disequilibrium test (TDT) and sib transmission-disequilibrium test (STDT) in 68 type 2 diabetes pedigrees (377 people).
RESULTSA total of 40 SNPs were identified in length of 8,936 bp, with an average of 1 in every 223 bp. The SNPs in CAPN10 gene did not distribute evenly and the SNPs in Chinese were different from those reported in Mexican American. There was no significantly statistical difference in the allele frequency of the 5 SNPs between case and control, and the haplotype frequencies in the two groups were not significantly different. No positive results was found in TDT and STDT analysis.
CONCLUSIONSThe SNP distribution of CAPN10 gene differs in different nationalities. The studied SNPs in CAPN10 gene may not be the major susceptibility ones of type 2 diabetes mellitus in Han people of Northern China.
