Effect of ionizing radiation on the expression of p16, cyclinD1 and CDK4 in mouse thymocytes and splenocytes.
- Author:
Gui-Zhi JU
1
;
Xiao-Mei WANG
;
Shi-Bo FU
;
Shu-Zheng LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cyclin D1; biosynthesis; Cyclin-Dependent Kinase 4; Cyclin-Dependent Kinase Inhibitor p16; biosynthesis; Cyclin-Dependent Kinases; biosynthesis; Dose-Response Relationship, Radiation; Flow Cytometry; Male; Mice; Mice, Inbred Strains; Proto-Oncogene Proteins; Radiation Dosage; Spleen; cytology; metabolism; radiation effects; Thymus Gland; cytology; metabolism; radiation effects; X-Rays
- From: Biomedical and Environmental Sciences 2003;16(1):47-52
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effect of ionizing radiation on the expression of p16, CyclinD1, and CDK4 in mouse thymocytes and splenocytes.
METHODSFluorescent staining and flow cytometry analysis were employed for the measurement of protein expression.
RESULTSIn time course experiments, it was found that the expression of p16 protein was significantly increased at 8, 24, and 48 h for thymocytes (P < 0.05, P < 0.01, and P < 0.05, respectively) and at 24 h for splenocytes (P < 0.05) after whole body irradiation (WBI) with 2.0 Gy X-rays. However, the expression of CDK4 protein was significantly decreased from 8 h to 24 h for thymocytes (P < 0.05-P < 0.01) and from 8 h to 72 h for splenocytes (P < 0.05-P < 0.01). In dose effect experiments, it was found that the expression of p16 protein in thymocytes and splenocytes was significantly increased at 24 h after WBI with 1.0, 2.0, and 4.0 Gy (P < 0.05-P < 0.01), whereas the expression of CDK4 protein was significantly decreased with 2.0 Gy for thymocytes (P < 0.05) and 0.5-6.0 Gy for splenocytes (P < 0.05-P < 0.01). Results also showed that the expression of CyclinD1 protein decreased markedly in both thymocytes and splenocytes after exposure.
CONCLUSIONThe results indicate that the expression of p16 protein in thymocytes and splenocytes can be induced by ionizing radiation, and the p16-CyclinD1/CDK4 pathway may play an important role for G1 arrest of thymocytes induced by X-rays.
