Schwann cells transplantation promoted and the repair of brain stem injury in rats.
- Author:
Hong WAN
1
;
Yi-Hua AN
;
Mei-Zhen SUN
;
Ya-Zhuo ZHANG
;
Zhong-Cheng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antimetabolites; Brain Injuries; therapy; veterinary; Brain Stem; injuries; Bromodeoxyuridine; Cell Transplantation; methods; veterinary; Electrophysiology; Rats; Rats, Wistar; Schwann Cells; transplantation
- From: Biomedical and Environmental Sciences 2003;16(3):212-218
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the possibility of Schwann cells transplantation to promote the repair of injured brain stem reticular structure in rats.
METHODSSchwann cells originated from sciatic nerves of 1 to 2-day-old rats were expanded and labelled by BrdU in vitro, transplanted into rat brain stem reticular structure that was pre-injured by electric needle stimulus. Immunohistochemistry and myelin-staining were used to investigate the expression of BrdU, GAP-43 and new myelination respectively.
RESULTSBrdU positive cells could be identified for up to 8 months and their number increased by about 23%, which mainly migrated toward injured ipsilateral cortex. The GAP-43 expression reached its peak in 1 month after transplantation and was significantly higher than that in the control group. New myelination could be seen in destructed brain stem areas.
CONCLUSIONThe transplantation of Schwann cells can promote the restoration of injured brain stem reticular structure.
