Inhibiting effects of Achyranthes bidentata polysaccharide and Lycium barbarum polysaccharide on nonenzyme glycation in D-galactose induced mouse aging model.
- Author:
Hong-Bin DENG
1
;
Da-Peng CUI
;
Jian-Ming JIANG
;
Yan-Chun FENG
;
Nian-Sheng CAI
;
Dian-Dong LI
Author Information
- Publication Type:Journal Article
- MeSH: Achyranthes; chemistry; Aging; physiology; Animals; Disease Models, Animal; Erythrocytes; Female; Galactose; chemistry; Learning; Lycium; chemistry; Memory; Mice; Mice, Inbred C57BL; Motor Activity; Polysaccharides; pharmacology; Superoxide Dismutase; pharmacology
- From: Biomedical and Environmental Sciences 2003;16(3):267-275
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the inhibiting effects and mechanism of achyranthes bidentata polysaccharide (ABP) and lycium barbarum polysaccharide (LBP) on nonenzyme glycation in D-galactose induced mouse aging model.
METHODSSerum AGE levels were determined by AGE-ELISA, MTT method was used to determine lymphocyte proliferation, IL-2 activity was determined by a bioassay method. Spontaneous motor activity was used to detect mouse's neuromuscular movement, latency of step-through method was used to examine learning and memory abilities of mouse, colormetric assay was used to determine hydroxyproline concentration in mouse skin, pyrogallol autoxidation method was used to determine superoxide dismutase (SOD) activity of erythrocytes.
RESULTSDecreased levels of serum AGE, hydroxyproline concentration in mouse skin and spontaneous motor activity in D-galactose mouse aging model were detected after treated with ABP or LBP, while lymphocyte proliferation and IL-2 activity, learning and memory abilities, SOD activity of erythrocytes, were enhanced.
CONCLUSIONSABP and LBP could inhibit nonenzyme glycation in D-galactose induced mouse aging model in vivo and ABP has a better inhibiting effect than LBP.
