Experimental study on inhibitory effect of rutin against platelet activation induced by platelet activating factor in rabbits.
- Author:
Wen-mei CHEN
1
;
Ming JIN
;
Wei WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biological Transport, Active; Blood Platelets; metabolism; Calcium; metabolism; Male; Platelet Activating Factor; antagonists & inhibitors; Platelet Activation; drug effects; Platelet Aggregation Inhibitors; pharmacology; Rabbits; Rutin; pharmacology; Serotonin; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2002;22(4):283-285
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the inhibitory effect and mechanism of rutin against platelet activating factor (PAF) induced platelet aggregation, 5-HT release and intra-platelet free calcium concentration.
METHODSThe rate of washed rabbit platelet (WRP) aggregation was measured by turbidimetry and O-phthaldialdehyde (OPT) fluoro-spectrophotometry (FSPM) was used to determine 5-HT content. The intraplatelet free calcium concentration was measured with Fura-2/AM FSPM assay.
RESULTSRutin in vitro was concentration-dependently inhibiting PAF (9.55 x 10(-9) mol/L) induced WRP aggregation, the IC50 of 5-HT release was 0.73, 1.13 mmol/L respectively and the intraplatelet free calcium concentration elevation evoked by PAF (4.78 x 10(-10) mol/L) were inhibited by 68.3, 136, 274, 545 mumol/L of rutin dose-dependently.
CONCLUSIONRutin could inhibit PAF induced platelet aggregation, 5-HT release and the increase of intraplatelet free calcium.
