Effect of buyang huanwu decoction and its active regions combination on brain heat shock protein 70 expression in gerbils after cerebral ischemia/reperfusion.
- Author:
Chang-qing DENG
1
;
Min WANG
;
Fu-yuan HE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; metabolism; Brain Ischemia; metabolism; Drugs, Chinese Herbal; pharmacology; Female; Gerbillinae; HSP70 Heat-Shock Proteins; biosynthesis; genetics; Male; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Reperfusion Injury; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2002;22(3):193-210
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the effects of Buyang Huanwu Decoction (BYHWD) and its active regions combination on brain heat shock protein 70 (HSP70) expression.
METHODSCerebral ischemia model gerbils produced by ligating bilateral common carotid artery were injected intraperitoneally by BYHWD and its active regions combination separately. HSP70 mRNA expression was determined by Northern blot test and protein expression determined by Western blot test 48 hrs after ischemia for 15 min and reperfusion.
RESULTSThe level of HSP70 mRNA and protein expression in ischemic/reperfusion brain tissue increased significantly. BYHWD and its active regions combination could decrease the HSP70 mRNA expression obviously, the inhibition effect of BYHWD was more significant than that of its active regions combination, but neither of them showed any effect on HSP70 protein expression.
CONCLUSIONBYHWD and its active regions combination might down-regulate HSP70 mRNA expression in ischemic brain tissue through its anti-ischemic effect.
