Effect of wuzi yanzong Pill on mitochondrial DNA deletion and respiratory chain enzyme complex activity in peripheral leukocyte of aged male with kidney deficiency syndrome.
- Author:
Xue-mei WANG
1
;
Hong FU
;
Geng-xin LIU
Author Information
- Publication Type:Clinical Trial
- MeSH: Aged; Aged, 80 and over; Aging; drug effects; DNA, Mitochondrial; genetics; Diagnosis, Differential; Drugs, Chinese Herbal; therapeutic use; Humans; Kidney Diseases; drug therapy; Leukocytes; metabolism; Male; Medicine, Chinese Traditional; Middle Aged; Phytotherapy; Sequence Deletion; drug effects; Yang Deficiency; drug therapy; genetics
- From: Chinese Journal of Integrated Traditional and Western Medicine 2002;22(2):101-103
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Wuzi Yanzong Pill (WZYZP) on mitochondrial DNA (mtDNA) deletion and respiratory chain enzyme complex (RCZC) in peripheral blood leukocyte of aged male with Kidney Deficiency Syndrome (KDS).
METHODSSingle-blinded study was conducted in 38 aged male with KDS, who were randomly divided into 2 groups treated with WZYZP and placebo respectively for 3 months. Levels of mtDNA deletion and RCZC were determined by polymerase chain reaction (PCR) and enzyme kinetics technique respectively.
RESULTSWZYZP could reduce the mtDNA deletion and raise the activity of mitochondrial RCZC I, IV in peripheral blood leukocyte of aged male with KDS (P < 0.05, P < 0.01).
CONCLUSIONWZYZP has protective effect on mtDNA from oxidative damage in leukocyte of aged male with KDS.
