Effect of 1, 2, 3, 4, 6, 7, 8, 9-octachlorodibenzo-p-dioxin on testicular gene expression profiles in male mice.
- Author:
Wei ZHANG
1
;
Yao-Feng WANG
;
Tian-Jun NI
;
Zhi-Jun YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Male; Mice; Mice, Inbred C57BL; Oligonucleotide Array Sequence Analysis; Polychlorinated Dibenzodioxins; toxicity; Testis; drug effects; metabolism; Transcriptome
- From: Journal of Southern Medical University 2016;36(3):316-320
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of 1,2,3,4,6,7,8,9-octachlorodibenzo-p-dioxin (OCDD) on the testicular gene expression profile in the testis of mice.
METHODSTwenty male C57BL/6j mice were randomly divided into normal control group (fed with maize oil) and 3 OCDD groups treated with OCDD by gavage for 30 days at low-, moderate-, and high doses of 1.25×10(-6), 1.25 ×10(-5), and 1.25×10(-4) g/mL, respectively (8 mL/kg daily). The testicular gene expression profiles of the mice were investigated using gene chip technique and compared between OCDD-exposed groups and the control group.
RESULTSCompared with the control group, the mice in low-dose OCDD group showed 1133 differentially expressed genes, including 659 up-regulated and 474 down-regulated ones; in the moderate-dose OCDD group, 978 genes were differentially expressed, including 487 up-regulated and 491 down-regulated ones; in the high-dose group, 895 genes were differentially expressed, including 424 up-regulated and 471 down-regulated ones.
CONCLUSIONThe effect of sub-chronic exposure to OCDD on testicular gene expression profiles in male C57BL/6j mice indicates that the testis is probably the target organ of OCDD.
