Effect of F10 gene silencing and over-expression on cell cycle of choriocarcinoma cell line JAR and the mechanisms.
- Author:
Jin-di YANG
1
;
Zhan-Jun PANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Cycle; Cell Division; Cell Line, Tumor; Cell Proliferation; Choriocarcinoma; metabolism; Cyclin-Dependent Kinases; metabolism; Cyclins; metabolism; Factor X; genetics; Female; Gene Silencing; Humans; Pregnancy
- From: Journal of Southern Medical University 2016;36(3):351-355
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the role of F10 gene in regulating cell cycles of choriocarcinoma cells and the underlying mechanisms.
METHODSUsing untreated cells as the control, JAR cells with F10 gene silencing or stable F10 over-expression were examined for cell cycle changes by flow cytometry (FCM) and for expressions of cyclin and cyclin-dependent kinase (CDKs) with Western blotting and immunofluorescence technique.
RESULTSJAR cells over-expressing F10 gene showed reduced duration of cell cycle compared with untreated and with cells after F10 gene silencing. In F10-over-expressing cells, Western blotting revealed significantly up-regulated expressions of cyclin A2, B1, D1, E and CDK2, 6, and 7, but not CDK4, as compared with the control cells and cells with F10 gene silencing (P<0.05), and these results were consistent with those by immunofluorescence assay.
CONCLUSIONF10 gene may accelerate cell cycle progression and promote cell proliferation by up-regulating the expressions of cyclin A2, B1, D1, E and CDK 2, 4, 6, 7 in choriocarcinoma cells.
