Effects of exendin-4 on extracellular matrix metabolism in human mesangial cells cultured in high glucose.
- Author:
Zhi-Zhou XIAO
1
;
Mei-Ping GUAN
;
Zong-Ji ZHENG
;
Yi-Jie JIA
;
Ling WANG
;
Yao-Ming XUE
Author Information
- Publication Type:Journal Article
- MeSH: Cell Proliferation; Cells, Cultured; Collagen Type I; metabolism; Culture Media; chemistry; Diabetic Nephropathies; Extracellular Matrix; metabolism; Fibronectins; metabolism; Glucose; chemistry; Humans; MAP Kinase Signaling System; Mesangial Cells; drug effects; Peptides; pharmacology; Phosphorylation; Signal Transduction; Transforming Growth Factor beta1; metabolism; Venoms; pharmacology
- From: Journal of Southern Medical University 2016;36(3):371-374
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore effects of exendin-4 on the metabolism of extracellular matrix (ECM) in human mesangial cells (HMC) cultured in the presence of high glucose and explore the possible mechanism.
METHODSHuman mesangial cells (HMC) were treated with exendin-4 under high glucose conditions. The cell proliferation was observed using CCK8 assay, and the expressions of collagen type I, fibronectin, transforming growth factor-β1 (TGFβ1) expression and extracellular signal- regulated kinase (ERK) signaling pathway activity were assessed using Western blotting.
RESULTSExendin-4 inhibited cell proliferation and the expressions of collagen type I, fibronectin and TGFβ1 and reversed ERK phosphorylation in high glucose-induced HMC.
CONCLUSIONExendin-4 can regulate ECM metabolism in HMC cultured in high glucose by inhibiting TGFβ1/ERK pathway, suggesting the beneficial effects of exendin-4 in preventing and treating diabetic nephropathy.
