TALEN-mediated MYH9 Knock-down and its influence on cell cycle and apoptosis of MGC803 cell line.
- Author:
Xian-Jun ZHU
1
;
Hai-Jun DENG
;
Geng-Tai YE
;
Zhi-Yong SHEN
;
Feng-Ping LI
;
Wei-Hong GUO
;
Qing-Bin YANG
;
Hao LIU
;
Guo-Xin LI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Gene Knockdown Techniques; Humans; Molecular Motor Proteins; genetics; Myosin Heavy Chains; genetics; Plasmids; Stomach Neoplasms; Transfection
- From: Journal of Southern Medical University 2016;36(3):375-380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct a MYH9 gene knockout model in MGC803 cell line using transcription activator-like effector nuclease (TALEN) and observe its effect on cell cycle and apoptosis.
METHODSAccording to FastTALE(TM) TALEN Kit, we designed TALEN pairs and constructed the plasmids targeting to MYH9 gene. After detecting their activity in MGC803 cells by plasmid transfection, DNA sequencing, RT-PCR and western blot, we selected the monoclonal cells and studied the changes in the cell cycle and apoptosis.
RESULTSMYH9 gene could not be knocked out but knocked down in selected MGC803 monoclonal cells, which caused cell cycle arrested at G2/M phase (P<0.05) and a significant increase in the cell number with early apoptosis (P<0.01).
CONCLUSIONWe successfully generated a MYH9 knockdown model in MGC803 cell lines by TALEN, which could be in favor of MYH9 function study in gastric cancer.
