Expression of Rictor and mTOR in colorectal cancer and their clinical significance.
- Author:
Li-Feng WANG
1
;
Hai-Jin CHEN
;
Jin-Long YU
;
Jia QI
;
Xiao-Hua LIN
;
Zhao-Wei ZOU
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Carrier Proteins; metabolism; Cell Line, Tumor; Colorectal Neoplasms; metabolism; Disease Progression; Humans; Immunohistochemistry; Lymphatic Metastasis; Prognosis; Rapamycin-Insensitive Companion of mTOR Protein; Survival Rate; TOR Serine-Threonine Kinases; metabolism
- From: Journal of Southern Medical University 2016;36(3):396-400
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the expression of Rictor and mTOR in the colorectal cancer and their clinical significance.
METHODSThe expression levels of Rictor and mTOR in HCT116, SW480, LoVo and HCoEpiC cells were detected by indirect immunofluorescence and Western blotting. Sixty-two paraffin-embedded surgical specimens of colorectal cancer tissue and adjacent tissues were examined for Rictor expression using immunohistochemistry. The association of the expression levels of Rictor protein with the clinicopathologic features and the overall survival of the patients was analyzed.
RESULTSThe expression level of Rictor was significantly higher in colorectal cancer tissues than in the adjacent tissues (P<0.05). The expression levels of Rictor and mTOR in the colon cancer cell lines were higher than those in human normal colon epithelial cell line HCoEpiC. The expression of Rictor was correlated with Dukes stage and lymphatic metastasis of the tumors but not with other clinicopathological parameter (P>0.05). Patients with Rictor expression had a lower overall survival rate than those without Rictor expression.
CONCLUSIONRictor overexpression is associated with the carcinogenesis and progression of colorectal cancer and can be an independent indicator for evaluating the prognosis of colorectal cancer patients.
