Nanog promotes the invasion of breast cancer cells by increasing PKCε expression.
- Author:
Wen-Feng SHAO
1
;
Fu-Xi LI
;
Rui TANG
;
Xiao-Ran YU
;
Qiao-Sheng WEN
;
Yan-Lin YU
;
Jing-Bo XIONG
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Breast Neoplasms; metabolism; Cytoskeletal Proteins; metabolism; Gene Knockdown Techniques; Homeodomain Proteins; metabolism; Humans; Nanog Homeobox Protein; Neoplasm Invasiveness; Phosphorylation; Protein Kinase C-epsilon; metabolism; RNA, Small Interfering; Transfection; Tumor Cells, Cultured; Up-Regulation
- From: Journal of Southern Medical University 2016;36(5):639-644
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the relationship between Nanog-promoted metastasis of breast cancer and ezrin(T567) phosphorylation, and explore the possible mechanism by which Nanog regulates ezrin(T567) phosphorylation.
METHODSA siRNA construct targeting Nanog was transfected in breast cancer cells to knock down Nanog expression, and the changes in the cell invasion was detected using Transwell assay. The expression levels of Nanog and PKC and the phosphorylation level of ezrin(T567) were detected using Western blotting and immunofluorescent staining; the protein interaction between PKCε and ezrin was assayed by co-immunoprecipitation and Western blotting.
RESULTSNanog knockdown significantly decreased the expression of PKCε protein, phosphorylation level of ezrin(T567) and the invasion ability of breast cancer cells. PKCε knockdown obviously decreased the phosphorylation level of ezrin(T567) in the cells, and PKCε and ezrin were co-immunoprecipitated.
CONCLUDIONSNanogcan can upregulate the expression of PKCε to promote the phosphorylation of ezrin(T567), which can be a new mechanism by which Nanog promotes tumor metastasis.
