Solanine inhibits prostate cancer Du145 xenograft growth in nude mice by inducing cell cycle arrest in G1/S phase.
- Author:
Wei-Feng ZHONG
1
;
Si-Ping LIU
;
Bin PAN
;
Zhao-Feng TANG
;
Jin-Guang ZHONG
;
Fang-Jian ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Cyclin-Dependent Kinases; metabolism; Cyclins; metabolism; G1 Phase Cell Cycle Checkpoints; Humans; Male; Mice; Mice, Nude; Neoplasm Transplantation; pathology; Prostatic Neoplasms; drug therapy; pathology; S Phase; Solanine; pharmacology
- From: Journal of Southern Medical University 2016;36(5):665-670
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of solanine on the growth of human prostate cancer cell xenograft in nude mice.
METHODSHuman prostate cancer Du145 cells were injected into the subcutaneous layers on the back of nude mice. After a week, the mice bearing subcutaneous tumor graft were randomly divided into solanine treatment group and saline control group for treatment for 3 weeks. The tumor grafts were then harvested to evaluate the inhibition rate. The mRNA and protein expressions of cell cycle-related genes in the tumors were detected by qRT-PCR and Western blotting, respectively, and tumor cell apoptosis was detected using TUNEL method.
RESULTSThe tumor growth rate in solanine-treated group was significantly slower than that in the control group (P<0.01). The mRNA and protein expressions of C-myc, cyclin D1, cyclin E1, CDK2, CDK4 and CDK6 were significantly inhibited by solanine. Solanine significantly up-regulated p21 mRNA and protein expression in the tumors and induced a higher apoptosis rate of the tumor cells than saline (P<0.01).
CONCLUSIONThe tumor-inhibition effect of solanine is probably mediated by regulating the expressions of genes related with G1/S cell cycle arrest and cell apoptosis.
