Effect of DAPK overexpression on biological behaviors and caspase-3 expression in HL-60 cells.
- Author:
Wei-Hua ZHAO
1
;
Fan-Yi MENG
;
Yong-Rong LAI
;
Zhi-Gang PENG
;
Jie MA
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Caspase 3; metabolism; Cell Cycle; Cell Differentiation; Cell Line, Tumor; Death-Associated Protein Kinases; metabolism; HL-60 Cells; Humans; RNA, Messenger; metabolism; Transfection; U937 Cells
- From: Journal of Southern Medical University 2016;36(5):729-732
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of DAPK overexpression on the biological behaviors and caspase-3 expression in HL-60 cells.
METHODSThe expression of DAPK mRNA was detected by RT-PCR leukemia cell lines K562, Molt4, U937, and HL-60 cells. HL-60 cells were transfected by a eukaryotic expression vector pReceiver-M29-DAPK via LipofectamineTM 2000, and the impact of DAPK overexpression on cell apoptosis, cell cycle, cell differentiation and caspase-3 expression were analyzed.
RESULTSDAPK mRNA expression was positive in K562, Molt4 and U937 cells but negative in HL-60 cells. Significantly increased cell apoptosis was observed in pReceiver-M29-DAPK-transfected HL-60 cells by flow cytometry and Hoechst33342 staining. The cell cycle distribution and differentiation showed no significant changes after the transfection. The expression of caspase-3 was significantly increased in the cells after transfection.
CONCLUSIONDAPK gene overexpression promotes apoptosis of HL-60 cells without affecting the cell cycle and differentiation. Caspase-3 may be involved in the regulation of cell apoptosis.
