Determination of plasma protein binding rate of vitexin.
- Author:
Chengliang TONG
1
;
Xiaoying WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apigenin; metabolism; Blood Proteins; metabolism; Chromatography, High Pressure Liquid; Dialysis; Humans; Protein Binding; Rats; Species Specificity
- From: China Journal of Chinese Materia Medica 2012;37(14):2168-2170
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an analytical method on vitexin concentration in plasma to determine the plasma protein binding rate of vitexin.
METHODThe equilibrium dialysis method and HPLC were adopted to determine vitexin concentration in plasma, calculate human plasma protein binding rate of vitexin and compare rat and human plasma protein binding rates of vitexin.
RESULTAt 2-16 mg x L(-1), there was no significant difference in the plasma protein binding rate. But the human plasma protein binding rate of vitexin was higher than its rat plasma protein binding rate, indicating a significant difference in rat and human plasma protein binding rates of vitexin.
CONCLUSIONVitexin has a higher protein binding rate with both rat plasma and human plasma.
