Detoxication experimental study on different compatibility proportion of Aconiti Lateralis Radix Praeparata and glycyrrhizae radix et rhizoma.
- Author:
Suhua XIE
1
;
Guangping ZHANG
;
Guibo SUN
;
Xiaoguang ZHU
;
Rongchang CHEN
;
Siyu ZHANG
;
Ling HE
;
Zuguang YE
Author Information
- Publication Type:Journal Article
- MeSH: Aconitum; chemistry; Animals; Cells, Cultured; Chemistry, Pharmaceutical; methods; Drug Interactions; Drugs, Chinese Herbal; administration & dosage; pharmacokinetics; toxicity; Female; Glycyrrhiza; chemistry; Inactivation, Metabolic; Male; Mice; Mice, Inbred ICR; Myocytes, Cardiac; drug effects; metabolism; Rats; Rats, Sprague-Dawley; Rhizome; chemistry
- From: China Journal of Chinese Materia Medica 2012;37(15):2210-2214
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThrough the paired comparison on the toxicity effect of Aconiti Lateralis Radix Praeparata of different compatibility proportion of Aconiti Lateralis Radix Praeparata and Glycyrrhizae Radix et Rhizoma, to observe the detoxication effect of Glycyrrhizae Radix et Rhizoma to Aconiti Lateralis Radix Praeparata.
METHODPaired comparison on the mouse acute toxicity of Aconiti Lateralis Radix Praeparata and Aconiti Lateralis Radix Praeparata with different compatibility proportion of Glycyrrhizae Radix et Rhizoma, to assay the LD50. Paired comparison on the rat heart toxicity of Aconiti Lateralis Radix Praeparata and Aconiti Lateralis Radix Praeparata with different compatibility proportion of Glycyrrhizae Radix et Rhizoma, to assay the TD50. We dilute medicated serum of Aconiti Lateralis Radix Praeparata, Aconiti Lateralis Radix Praeparata plus Glycyrrhizae Radix et Rhizoma (3:1), Aconiti Lateralis Radix Praeparata plus Glycyrrhizae Radix et Rhizoma (1: 1) into 5%, 10%, 20% solution with serum free DMEM, to survey the effect of different concentration of medicated serum to the pulsing rhythm of myocardial cell of original generation newborn rat, cell surviva rate and content of LDH in myocardial cells.
RESULTLD50 and TD50 of Aconiti Lateralis Radix Praeparata can be increased after adding Glycyrrhizae Radix et Rhizoma Compared to the blank serum, medicated serum with Aconiti Lateralis Radix Praeparata can obviously increased the pulse rhythm of myocardial cell and the content of LDH (P < 0.05). The medicated serum with Aconiti Lateralis Radix Praeparata added different proportion of Glycyrrhizae Radix et Rhizoma can reduce the acceleration of myocardial cell's rhythm, which is induced by Aconiti Lateralis Radix Praeparata, and can reduce the content of LDH. With the increased ratio of Glycyrrhizae Radix et Rhizoma, the effect is stronger. But for the serum with different concerntration of Aconiti Lateralis Radix Praeparata or Aconiti Lateralis Radix Praeparata added Glycyrrhizae Radix et Rhizoma, there is no obvious effect to the cell survival.
CONCLUSIONGlycyrrhizae Radix et Rhizoma has the detoxication effect through increasing the ultimatetotaldosage of Aconiti Lateralis Radix Praeparata. The detoxication effect of Glycyrrhizae Radix et Rhizoma to Aconiti Lateralis Radix Praeparata is through restraining the increased rhythm of myocardial cell and protecting the myocardial cell.
