Optimization of biotransformation of ursolic acid by alternaria longipes.
- Author:
Huijun YAN
1
;
Xuan WANG
;
Ying GAO
;
Dailin LIU
Author Information
- Publication Type:Journal Article
- MeSH: Alternaria; chemistry; metabolism; Biotransformation; Culture Media; metabolism; Culture Techniques; methods; Hydrogen-Ion Concentration; Temperature; Triterpenes; analysis; metabolism
- From: China Journal of Chinese Materia Medica 2012;37(15):2280-2285
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo conduct the biotransformation of ursolic acid by alternaria longipes AS3. 2875, and optimize the culture medium and biotransformation conditions.
METHODWith the consumption rate of ursolic acid and the generation rate of 28-O-beta-D-glucopyranose ursolic acid as indicators, the impact of different biotransformation conditions such as pH, phosphate, different kinds of metal ions, spore concentration, substrate quantity, temperature, shaking speed and cultivation time on the transformation of ursolic acid in alternaria longipes culture solution were detected to obtain the optimal biotransformation conditions of ursolic acid by alternaria longipes.
RESULTThe optimized biotransformation conditions were as follows: initial pH value was 5.0, MgSO4 was 0.25 g x L(-1) K2HPO4 was 1.0 g x L(-1), FeSO4 was 0.083 g x L(-1), spore concentration was 4%, substrate quantity was 0.3 g x L(-1), shaking speed was 140 r x min(-1), cultivation temperature was at 28 degrees C and cultivating time was 3 days.
CONCLUSIONThe generation rate of 28-O-beta-D-glucopyranose ursolic acid by alternaria longipes stabilizes at around 5%.
