Analysis on chemical components of Ligustrum lucidum by ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry.
- Author:
Yejuan JIANG
1
;
Weifeng YAO
;
Li ZHANG
;
Anwei DING
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; isolation & purification; Ligustrum; chemistry; Molecular Structure; Spectrometry, Mass, Electrospray Ionization; methods
- From: China Journal of Chinese Materia Medica 2012;37(15):2304-2308
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo make a qualitative analysis on chemical components in Ligustrum lucidum by UPLC-ESI-Q-TOF-MS.
METHODACQUITY UPLC BEH C18 (2.1 mm x 100 mm, 1.7 microm) column was adopted, with methyl cyanides-0.1% formic acid as the mobile phase for gradient elution; ESI ion source was used for mass spectra, and data were collected in positive and negative mode.
RESULTFourteen compounds of L. lucidum were identified by analyzing positive and negative ion mass spectra information and element composition and comparing controls with data from relevant literature.
CONCLUSIONAfter the separation by ultra high performance liquid chromatography, relative molecular mass was determined by mass spectra, and chemical compounds in L. lucidum were determined by information search through relevant literature data, in order to provide powerful therapeutic material basis for L. lucidum.
