Determination of nine steroidal saponins in Paris polyphylla from different areas of Guizhou province by HPLC.
- Author:
Yuyong LIANG
1
;
Zhen LIU
;
Wenyuan GAO
;
Shuli MAN
Author Information
- Publication Type:Journal Article
- MeSH: China; Chromatography, High Pressure Liquid; methods; Liliaceae; chemistry; Phytosterols; analysis; isolation & purification; Plant Extracts; analysis; isolation & purification; Rhizome; chemistry; Saponins; analysis; isolation & purification
- From: China Journal of Chinese Materia Medica 2012;37(15):2309-2312
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare steroidal saponins-main active ingredients of Paris polyphylla from different areas in Guizhou province, in order to provided basis for further proving Guizhou province to be the planting base of P. polyphylla.
METHODThe contents of nine steroidal saponins, namely paris-VII, (25R)-5-en-spirost-3beta,17alpha-diol-3-O-alpha-L-rhamnopyranosyl-(1-->2) [alpha-L-rhamnopyranosyl(1-->4)]-beta-D-glycopyanoside (PGRR), paris-H, paris-VI, paris-II , paris-III, gracillin, paris-I and paris-V of P. polyphylla from eight areas of Guizhou province were determined by HPLC-UV.
RESULTFive steroidal saponins-paris-VII, PGRR, paris-H, paris-VI and gracillin were detected in all the drugs, which provided basis for distinguishing P. polyphylla. P. polyphylla from Dushan showed the highest content of steroidal saponins (9.62%), followed by Tongren (6.39%), and the lowest was Zunyi (0.99%).
CONCLUSIONSaponins from different areas of Guizhou province show significant difference in variety and content, therefore Dushan is suitable for planting P. polyphylla.
