Determination of carboxyatractyloside and atractyloside in Xanthii Fructus by HPLC.
- Author:
Rui DUO
1
;
Yan CHEN
;
Yuhong LIU
;
Zhifang HUANG
;
Yunhua LIU
;
Jinhai YI
Author Information
- Publication Type:Journal Article
- MeSH: Asteraceae; chemistry; Atractyloside; analogs & derivatives; analysis; isolation & purification; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; isolation & purification; Fruit; chemistry
- From: China Journal of Chinese Materia Medica 2012;37(15):2313-2316
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine carboxyatractyloside and atractyloside in Xanthii Fructus by HPLC.
METHODBy HPLC, Agilent ZORBAX SB-phenyl (4.6 mm x 250 mm, 5 microm) column was adopted, with acetonitrile-0.01 mol x L(-1) NaH2PO4 (pH 6) as the mobile phase for gradient elution at the flow rate of 1.0 mL x min(-1). The detection wavelength was 203 nm, and the temperature was set at 35 degrees C.
RESULTCarboxyatractyloside showed a good linearity within the range of 0.0972-1.944 microg and atractyloside showed a good linearity within the range of 0.1030-2.060 microg. The recovery rate of carboxyatractyloside was 100. 3% and that of atractyloside was 102.5%. The RSD were 0.67% and 1.4% (n=6).
CONCLUSIONThis method is so simple, practical and highly repeatable that is can be used for quality control of Xanthii Fructus.
