Chemical constituents from Hypericum perforatum.
- Author:
Jie MA
1
;
Jianbo YANG
;
Tengfei JI
;
Aiguo WANG
;
Yalun SU
Author Information
- Publication Type:Journal Article
- MeSH: Anthraquinones; chemistry; isolation & purification; Drugs, Chinese Herbal; chemistry; isolation & purification; Glucosides; chemistry; isolation & purification; Hypericum; chemistry; Molecular Structure; Norisoprenoids; chemistry; isolation & purification
- From: China Journal of Chinese Materia Medica 2012;37(16):2408-2412
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the chemical constituents from Hypericum perforatum.
METHODCompouds were isolated by chromatographic techniques. Their structures were identified by spectral methods. The inhibitory activity of recombinant human PTP1B was evaluated.
RESULTNine compounds were elucidated as D-Mannitol (1), 1,2-benzenedicarboxylic acid bis(1-methylpropyl) ester (2), (7E, 6R,9S)-9-hydroxy-4,7-megastigmadien-3-one (3), (6S,9R)-roseoside (4) , 2,6-dimethoxy-4-hydroquinone-l-O-beta-D-glucopyranoside (5), 2,6-di-methoxy-4-hydroxybenzyl alcohol 1 -O-beta-D-glucopyranoside (6), syringate 4-O-beta-glucopyranoside (7), hypericin (8), skyrin-6-0-beta-D-glucopyranoside (9) , (R)-2,3-dihydroxypropyl 3,4-dihydroxy-benzoate (10). At a concentration of 2 micromol x L(-1), compound 8 inhibited recombinant human PTP1B with inhibitory rate of 96.4% and IC50 of 2.5 micromol x L(-1).
CONCLUSIONcompound 10 was new, compounds 2-4, 7 were obtained from Hypericum for the first time and compounds 5-6 was isolated from this plant for the first time. Compound 8 showed remarkable PTP1B inhibitory activity.
