Simultaneous determination of eight iridoid glycosides in Gardeniae fructus by HPLC.
- Author:
Wuzhan LIU
1
;
Jianwei FAN
;
Yanhong GAO
;
Xinjian QIU
;
Huifang ZHUANG
;
Zhiquan ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; isolation & purification; Gardenia; chemistry; Iridoid Glycosides; analysis; isolation & purification
- From: China Journal of Chinese Materia Medica 2012;37(16):2417-2421
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an HPLC method for simultaneous determination of eight iridiods in Gardeniae Fructus.
METHODKromasil C18 column (4. 6 mm x 250 mm, 5 microm) was adopted, with acetonitrile-water-trifluoroacetic acid (6:94: 0.05) as the mobile phase at the flow rate of 1.0 mL x min(-1). The detection wavelength was set at 238 nm, and the column temperature was 40 degrees C.
RESULTThe linear ranges of geniposide, gardoside, shanzhiside, geniposidic acid, deacetyl asperulosidic acid methyl ester, gardenoside, scandoside methyl ester, and genipin gentiobioside were 1.5036 - 15.036, 0.04256 - 0.4256, 0.1038 - 1.038, 0.00992 - 0.0992, 0.02332 - 0.2332, 0.4128 - 4.128, 0.02040 - 0.2040 and 0.4656 - 4.656 microg, respectively. Their average recoveries were 99.6% , 100.6% , 101.2%, 99.5%, 100.3% , 98.7%, 99.8% and 100.1%, respectively.
CONCLUSIONThe method shows good separation and it is so simple, accurate and highly repeatable that it can be used for providing basis for quality control of Gardeniae Fructus.
