Study on establishment of kidney deficient aging model and comparison with D-galactose induced aging model.
- Author:
Zhan LI
1
;
Renhui LIU
;
Xue KANG
;
Xiujuan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Aging; drug effects; physiology; Animals; Antioxidants; metabolism; Disease Models, Animal; Galactose; adverse effects; Humans; Kidney; physiopathology; Kidney Diseases; chemically induced; metabolism; physiopathology; Liver; enzymology; Male; Malondialdehyde; metabolism; Oxidative Stress; Rats; Rats, Sprague-Dawley; Superoxide Dismutase; metabolism
- From: China Journal of Chinese Materia Medica 2012;37(16):2435-2438
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a kidney deficient aging model (KDAM), assess it in antioxidant capacity, HPAT axis function and bone metabolism, and compare with D-galactose aging model.
METHODAging rat model was established by injecting D-galactose solution, meanwhile dexamethasone solution was injected to establish kidney deficient aging model. Then these models were evaluated by serum MDA (malondialdehyde) and GSH-Px (glutathione peroxidase), liver SOD (superoxide dismutase), adrenal, thymus and spleen index, CD4(+), CD8(+), and serum COR (cortisol), BGP (bone Gla-protein), plasma ACTH (adrenocorticotropic hormone) and CRH (corticotropin-releasing hormone).
RESULTCompared with the normal group, the aging model group and the kidney deficient aging group showed significant decrease in liver SOD activity (P < 0.01 on average) and significant increase in serum MDA content (P < 0.01 on average) , and the kidney deficient aging group revealed remarkable decline in plasma ACTH content (P < 0.05). Compared with the normal group and the aging model group, the kidney deficient aging model group's weight, serum GSH-Px decreased (P < 0.01, P < 0.05), adrenal index decreased (P < 0.05, P < 0.01), serum COR decreased (P < 0.05 on average), plasma CRH increased (P < 0.05, P < 0.01), serum BGP content significantly decreased (P < 0.01 on average), value of CD4(+), CD8(+) decreased (P < 0.05, P < 0.01), CD4(+)/CD8(+) increased, but without significant difference.
CONCLUSIONThe kidney deficient aging model shows significant decrease in antioxidant capacity, dysfunction of HPAT axis disorder and abnormal bone metabolism. However, D-galactose aging model only shows a significant difference in antioxidant capacity.