Determination method of muscone in rat intestinal perfusate by GC-MS/MS and its intestinal absorption kinetic characteristics in rats.
- Author:
Liang ZOU
1
;
Junzhi LIN
;
Zhanguo WANG
;
Lijia XU
;
Ping WANG
;
Gang ZHAO
;
Jieying LUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chromatography, Gas; methods; Cycloparaffins; analysis; pharmacokinetics; Intestinal Absorption; drug effects; Intestines; drug effects; metabolism; Perfusion; Rats; Rats, Wistar; Tandem Mass Spectrometry; methods
- From: China Journal of Chinese Materia Medica 2012;37(16):2456-2460
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the method for determining muscone in rat intestinal perfusate by GC-MS/MS and study its intestinal absorption kinetic characteristics in rats.
METHODThe GC-MS/MS method was used to determine the content of muscone in rat intestinal circulation fluid. In situ intestinal circulation perfusion was adopted to study absorption kinetics of muscone in rats.
RESULTMuscone was proved to be well absorbed in each section of small intestine. Its absorption rate constants (Ka) and the absorption rate (A) in the rat intestine showed duodenum > jejunum (P < 0.05) , duodenum > ileum (P < 0.01). Its Ka, A and t1/2 in rat small intestine was 0.990 h(-1) , 43.58% and 0.705h, respectively.
CONCLUSIONMuscone was well absorbed in each intestinal section, with duodenum better than jejunum (Ka, T1/2, P < 0.05) significantly better than ileum (Ka, T1/2, P < 0.01; A, P < 0.05). There is no obvious statistical difference between jejunum and ileum.
