DNA identification of Zijingpi's adulterant species Schisandra sphenanthera based on NCBI nucleotide database analysis.
- Author:
Xiao-li CHENG
1
;
Cai-li LIAO
;
Chun-sheng LIU
;
Zhen-fang BAI
;
Yao-jun YANG
;
Jian ZHENG
;
Ji ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: DNA, Plant; genetics; Databases, Nucleic Acid; Drugs, Chinese Herbal; chemistry; standards; Molecular Sequence Data; Phylogeny; Quality Control; Schisandra; classification; genetics; Sequence Analysis, DNA
- From: China Journal of Chinese Materia Medica 2012;37(17):2534-2537
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo provide basis for quality control of Zijingpi, DNA identification was used based on NCBI nucleotide database analysis.
METHODFirstly, total DNA of Zijingpi was extracted. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and PCR products was directly sequenced after purification. Finally, ITS sequence similarity and phylogenetic tree were used for identification.
RESULTThe ITS sequence information of the mainstream commercial drugs of Zijingpi was obtained.
CONCLUSIONIt is firstly reported that the mainstream commercial drugs of Zijingpi was the bark of Schisandra sphenanthera.
