Study on fingerprint-pharmacology correlation of protective effect of Polygonum orientale on myocardial cell oxidative injury induced by H202.
- Author:
Lin ZHENG
1
;
Jing LI
;
Hui CHEN
;
Yong-lin WANG
;
Ai-min WANG
;
Yong HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Drugs, Chinese Herbal; analysis; pharmacology; Humans; Hydrogen Peroxide; toxicity; Myocytes, Cardiac; drug effects; metabolism; Oxidative Stress; drug effects; Polygonum; chemistry; Protective Agents; analysis; pharmacology
- From: China Journal of Chinese Materia Medica 2012;37(17):2585-2588
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the protective effect of flowers, branch with leaves and main stem of Polygonum orientale on H9c2 myocardial cells oxidative injury induced by H202 and discuss its efficacious substance foundation of anti-myocardial ischemia.
METHODUPLC fingerprint was established for flowers, branch with leaves and main stem of P. orientale, and the chromatographic peak was identified. Meanwhile, the protective effect of composites of flowers, branch with leaves and main stem of P. orientale in different proportions on myocardial cells oxidative injury was observed (with MTT method as indicators of concentration screening and cell livability) and MDA were detected. The correlation analysis was made between activity information of composites and chemical information of UPLC fingerprint to infer efficacious substance foundation.
RESULTBranch with leaves and flowers from Polygonum orientale showed a protective effect on H9c2 myocardial cells oxidative injury. According to the study on spectrum-activity relationship, peak 3-5, 11-14, 18-19, 21-25 showed a positive correlation with their antioxidant activities.
CONCLUSIONThis study determined activity of Polygonum orientale on anti-oxidative injury, laying an experimental foundation for in-depth study and development of P. orientale herbs.
