Protective effect of paeonol on neurotoxicity induced by Abeta1-42 and underlying mechanisms.
- Author:
Shu-zhi ZHONG
1
;
Shi-ping MA
;
Quan-hai WANG
;
Zong-yuan HONG
Author Information
- Publication Type:Journal Article
- MeSH: Acetophenones; pharmacology; Alzheimer Disease; drug therapy; genetics; metabolism; physiopathology; Amyloid beta-Peptides; toxicity; Animals; Apoptosis; drug effects; Cell Line; Cells, Cultured; Hippocampus; cytology; drug effects; Humans; Neurons; drug effects; Neuroprotective Agents; pharmacology; Peptide Fragments; toxicity; Proto-Oncogene Proteins c-bcl-2; genetics; metabolism; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2012;37(17):2603-2606
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of paeonol on amyloid beta1-42 (Abeta1-42)-induced neurotoxicity and its mechanism.
METHODHippocampal neurons of well-grown newborn SD rats and differentiated SH-SY5Y cell lines were cultured with various concentrations of paeonol (1, 5, 10 micromol x L(-1), respectively) for 6 hours and then incubated with Abeta1-42 oligomer (30 micromol x L(-1)) for 24 hours and 48 hours, respectively. The neuron apoptosis was observed by Heochst33258. Annexin V/PI double stain flow cytometry assay was adopted for determining SH-SY5Y cell apoptosis rate. And the expression of BDNF and Bcl-2 mRNA was detected by RT-PCR.
RESULTCompared with the model group, various concentrations of paeonol (1, 5, 10 micromol x L(-1)) significantly reduced the hippocampal neurons karyopycnosis, decreased the rate of SH-SY5Y cell apoptosis to 22.4%, 18.1% and 16.4%, respectively, and improved the expressions of BDNF and Bcl-2 mRNA.
CONCLUSIONPaeonol relieves Abeta1-42 oligomer-induced neuron injury by increasing BDNF and Bcl-2 expressions.
