Anti-inflammatory effect and mechanism of artemisinin and dihydroartemisinin.
- Author:
Wan-ying YU
1
;
Wei-juan KAN
;
Peng-xia YU
;
Min-min LI
;
Ji-shuai SONG
;
Feng ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Inflammatory Agents; pharmacology; Artemisinins; pharmacology; Cell Line; Gene Expression; drug effects; Inflammation Mediators; immunology; Interleukin-6; genetics; immunology; Macrophages; drug effects; immunology; Mice; Nitric Oxide; immunology; Tumor Necrosis Factor-alpha; genetics; immunology
- From: China Journal of Chinese Materia Medica 2012;37(17):2618-2621
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study and compare the anti-inflammatory effect and molecular mechanism of artemisinin and dihydroartemisinin.
METHODMouse mononuclear macrophage RAW264.7 cells were stimulated to release inflammatory mediators such as TNF-alpha, IL-6 and NO, in order to assess the drugs' inhibitory effect on macrophage's release of above inflammatory mediators. The levels of TNF-alpha and IL-6 were determined by ELISA and the cytotoxicity was determined by MTT method. The protein expression of iNOS, COX-2 and beta-actin were tested by Western blot. The enzymatic activity of COX-2 was determined by colorimetric method.
RESULTDihydroartemisinin significantly inhibited LPS-induced release of TNF-alpha, IL-6 and NO from RAW264.7 in mice with the concentration range of 12.5 - 100 micromol x L(-1), and showed good dose dependence. Artemisinin only inhibited the IL-6 release to a certain extent.
CONCLUSIONDihydroartemisinin inhibits macrophages from releasing inflammatory factors TNF-alpha and IL-6 and inflammatory mediators NO by down-regulating iNOS protein. Artemisinin may help dihydroartemisinin to show its anti-inflammatory effect through metabolism.
