Genetic polymorphism of 6 short-tandem repeat loci in Miao minority group of Rongshui county in Guangxi province.
- Author:
Li-ning ZHOU
1
;
Lin XU
;
Ji-chun GONG
;
Song-feng LI
;
Qiong-ying DENG
;
Xiang-fa DENG
;
Hong-rong YU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Child; China; Ethnic Groups; genetics; Gene Frequency; Humans; Linkage Disequilibrium; Microsatellite Repeats; genetics; Polymerase Chain Reaction; Polymorphism, Genetic
- From: Chinese Journal of Medical Genetics 2005;22(6):709-711
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the distributions of six short-tandem repeat (STR) loci, namely D7S820, D13S317, D16S539, HUMCSF1PO, HUMTPOX and HUMTH01, in Miao minority group at Rongshui county in Guangxi province and construct the relevant genetic database.
METHODSSodium-citrated blood specimens were collected from 208 healthy unrelated Miao individuals in Rongshui county. The DNAs from the specimens were extracted with phenol-chloroform method; AmplFSTR Identifier PCR Amplification Kit was used to amplify the extracted DNAs, and 3100 Genetic Analyzer was used to analyze and screen the amplified products.
RESULTSIn this study, 7, 8, 6, 7, 5, 7 alleles were observed at the 6 STR loci respectively. The expected distribution of genotype accorded with Hardy-Weinbery equilibrium. The total discrimination power, cumulative paternity exclusion power and total polymorphism information were 0.999995, 0.9959 and 0.9987 respectively.
CONCLUSIONThe results demonstrate that these 6 STR loci are of high polymorphism and hereditary stability and are in accord with Mendel's law. The data obtained are valuable in population genetics research, forensic application, and individual identifications.
