Sequence analysis of a novel HLA-DRB1 allele, DRB1 * 1212.
- Author:
Fa-ming ZHU
1
;
Wei ZHANG
;
Qin-feng LU
;
Ji HE
;
Wei WANG
;
Zhe-dong HAN
;
Li-xing YAN
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Asian Continental Ancestry Group; genetics; Base Sequence; China; ethnology; DNA; analysis; Ethnic Groups; Female; HLA-DR Antigens; genetics; HLA-DRB1 Chains; Humans; Male; Molecular Sequence Data; Polymerase Chain Reaction; Sequence Analysis
- From: Chinese Journal of Medical Genetics 2006;23(1):47-49
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the molecular genetics basis for HLA novel allele HLA-DRB1*1212 in Chinese population.
METHODSGenomic DNA was extracted from whole blood by salting-out method. HLA-DRB1 gene exon 2 was amplified by PCR with group-specific primers from genomic DNA. PCR products were cut back from agarose gels and purified to sequence directly. The polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSO) was performed to confirm the mutations which were detected by sequencing in this study.
RESULTSThe sequencing results showed HLA-DRB1 alleles of the proband as DRB1*090102 and the novel allele. The sequences of the novel allele have been submitted to GenBank (AY899825). Through BLAST analysis, the novel allele was found to be different from DRB1*120101 at position 199A-->C in exon 2, that results in an amino acid change from Ile to Leu at codon 67.
CONCLUSIONThis allele is a novel and has been officially named as DRB1*1212 by the WHO Nomenclature Committee.