HLA-A, B, DRB1 gene polymorphism of Beijing population was studied by high-resolution polymerase chain reaction sequence-based typing.
- Author:
Ya-jun DENG
1
;
Guang YANG
;
Dong-ying WU
;
Song-nian HU
;
Sheng-bin LI
;
Jun ZHU
;
Bo-feng ZHU
;
Yao LIU
Author Information
- Publication Type:Journal Article
- MeSH: Asian Continental Ancestry Group; genetics; China; ethnology; Female; Genetics, Population; HLA-A Antigens; genetics; HLA-B Antigens; genetics; HLA-DR Antigens; genetics; HLA-DRB1 Chains; Humans; Male; Polymerase Chain Reaction; Polymorphism, Genetic; Population
- From: Chinese Journal of Medical Genetics 2006;23(1):103-106
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the gene polymorphism of human leukocyte antigen (HLA)-A, B, DRB1 loci in the population of Beijing region, and research on the application feasibility of polymerase chain reaction sequence-based typing (PCR-SBT) method.
METHODSPCR-SBT method was applied to determine HLA- A, B, DRB1 genotypes of 618 unrelated healthy individuals of Beijing region.
RESULTSA total of 84 different alleles and 199 genotypes of HLA-A, 143 alleles and 366 genotypes of HLA-B, 122 alleles and 286 genotypes of HLA-DRB1 were detected.
CONCLUSIONThe results showed the characteristics of HLA-A, B, DRB1 distributions, and provided more comprehensive and accurate gene data that may serve as normal reference values for all of Beijing people.
