With genechip technique to investigate HLA-DQB1 gene polymorphisms in south and north regions of China.
- Author:
Hui WANG
1
;
Da-lin WU
;
A-dong SHEN
;
Chun-yan CHEN
;
Wen ZHANG
;
Dan LI
;
Ya-qin LIU
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Asian Continental Ancestry Group; genetics; China; ethnology; Female; Gene Frequency; Genetic Variation; HLA-DQ Antigens; genetics; HLA-DQ beta-Chains; Humans; Male; Oligonucleotide Array Sequence Analysis; methods; Oligonucleotide Probes; Polymerase Chain Reaction; Polymorphism, Genetic; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2006;23(2):238-239
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo research and compare HLA-DQB1 gene frequency(GF) and polymorphism distribution between south and north population of Chinese in China.
METHODSCombining PCR-sequence specific primers(SSP) and sequence specific oligonucleotide probe(SSOP) techniques and DNA Microarray Kit for HLA-DQB1 Low Res Genotyping from Shenzhen Yi-Shengtang Biological LTD. Co. was used to type HLA-DQB1 gene polymorphisms of 700 individuals living in south China and 320 individuals in north China.
RESULTSWe inspected 10 alleles of HLA-DQB1 and got a series of comprehensive and accurate statistic data.
CONCLUSIONIt is tested that HLA-DQB1*02, 05, 0601, 0602, 0603 gene frequencies are different obviously(P<0.05) between south and north Chinese. And those data will be useful to kinds of research associated with disease relevant and anthropology research.
