Study on association of CTLA4 gene polymorphism with Grave's disease in Guangxi Zhuang nationality population.
- Author:
Xing-huan LIANG
1
;
Ying-fen QIN
;
Yan MA
;
Xin-rong XIE
;
Kai-qing XIE
;
Zuo-jie LUO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Antigens, CD; genetics; Base Sequence; CTLA-4 Antigen; China; Dinucleotide Repeats; genetics; Female; Genetic Predisposition to Disease; genetics; Graves Disease; genetics; Humans; Male; Middle Aged; Molecular Sequence Data; Polymerase Chain Reaction; Polymorphism, Genetic; genetics; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2006;23(3):306-309
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between the polymorphic (AT)n repeats in 3ountranslated region of exon 4 of CTLA4 gene [CTLA4(AT)n] and Graveso disease (GD) in Zhuang nationality population of Guangxi province.
METHODSThe studied groups comprised 48 patients with GD and 44 normal controls. Amplification of target DNA was carried out by polymerase chain reaction (PCR). The amplified products were run by 8% polyacrylamide gel electrophoresis, and then followed by 0.1% silver staining. Some of amplified products were sequenced directly.
RESULTSNineteen alleles of CTLA4 gene microsatellite polymorphism were found in Guangxi Zhuang nationality individuals. The 106 bp long allele was apparently increased in patients with GD of Zhuang nationality but not in healthy controls (P< 0.05).
CONCLUSIONCTLA4 gene microsatellite polymorphism is strongly associated with Graveso disease in Zhuang nationality population of Guangxi province. CTLA4(AT)n 106 bp may be the susceptible gene in GD patients of Zhuang nationality in Guangxi; 19 alleles of CTLA4 gene microsatellite polymorphism were found in Guangxi Zhuang nationality individuals.
