The investigation of STK15 gene amplification and overexpression in laryngeal squamous cell carcinoma.
- Author:
Yan YE
1
;
Fu-cai LI
;
Shu-yun WANG
;
Chan-yuan LI
;
Hai-ming YUAN
;
Xu ZHAO
;
Wei-neng FU
;
Zhen-ming XU
;
Kai-lai SUN
Author Information
- Publication Type:Journal Article
- MeSH: Aurora Kinase A; Aurora Kinases; Carcinoma, Squamous Cell; genetics; metabolism; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Laryngeal Neoplasms; genetics; metabolism; Protein-Serine-Threonine Kinases; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Medical Genetics 2006;23(3):326-329
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of STK15 gene amplification and overexpression to genesis and development of laryngeal squamous cell carcinoma (LSCC).
METHODSSTK15 gene amplification in 40 cases carcinoma tissues and normal tissues as control was detected by differential PCR approach. STK15 mRNA and protein levels were evaluated by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry method.
RESULTSIn 40 LSCC cases, STK15 gene amplification was found in 14 tumor tissues(35%), mRNA overexpression in 27 tumor tissues(67.5%), and protein upregulated in 29 tumor tissues(72.5%). Statistics analysis showed that STK15 gene amplification and mRNA overexpression were obviously associated to differentiation degree of LSCC, and protein overexpression was closely associated with both differentiation degree and pathological grades of LSCC.
CONCLUSIONThis research results suggest that STK15 gene amplification contributes to its mRNA and protein overexpression through affecting the exact replication of centrosome and separation of chromosomes. STK15 gene thus plays a role in LSCC oncogenesis and malignant progression.
