Allelic polymorphism analysis of killer cell immunoglobulin-like receptor gene 3DL2 in Zhejiang Han population.
- Author:
Kan JIANG
1
;
Fa-ming ZHU
;
Wei ZHANG
;
Qin-feng LU
;
Ji HE
;
Li-xing YAN
Author Information
- Publication Type:Journal Article
- MeSH: Asian Continental Ancestry Group; genetics; China; Gene Frequency; Genetics, Population; Humans; Polymerase Chain Reaction; Polymorphism, Genetic; genetics; Receptors, KIR3DL2; genetics; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2006;23(3):339-342
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the allelic polymorphism of killer cell immunoglobulin (Ig)-like receptor (KIR) gene 3DL2 in Zhejiang Han population.
METHODSAllelic definition within KIR3DL2 was achieved through using the PCR-sequence specific oligonucleotide probes (PCR-SSOP) method and the specific amplification of exon 3-exon 4 and exon 8-exon 9. Twenty-one digoxigenin-labelled probes were used to the SSOP analysis.
RESULTSSeven alleles of KIR3DL2 were observed, in which KIR3DL2 *002 had the highest allele frequency, 0.57. One sample could not be given the allele combination according to the probe profile.
CONCLUSIONThe PCR-SSOP method was reliable. There is a distinctive allele frequency of KIR3DL2 in Zhejiang Han population.
