Distribution of MIF -173 single nucleotide polymorphism checked by tetra-primer amplification refractory mutation system.
- Author:
Bao-ying FEI
1
;
Huo-xiang LU
;
Jian-min YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Alleles; Asian Continental Ancestry Group; genetics; Base Sequence; China; DNA Mutational Analysis; methods; Female; Gene Frequency; Genotype; Humans; Intramolecular Oxidoreductases; genetics; Macrophage Migration-Inhibitory Factors; genetics; Male; Middle Aged; Molecular Sequence Data; Mutation; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; genetics
- From: Chinese Journal of Medical Genetics 2006;23(3):343-345
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the distribution of macrophagemigration inhibitory factor gene (MIF) -173 single nucleotide polymorphism (SNP) of Chinese Han population in Zhejiang province.
METHODSThe DNA samples were extracted from EDTA blood of 142 unrelated healthy individuals. Alleles of MIF -173 SNP were genotyped by using the techniques of tetra-primer amplification refractory mutation system (ARMS) and restriction fragment length polymorphisms (RFLP)-PCR Meantime the PCR products were cloned and sequenced.
RESULTSThe authors detected three kinds of genotypes at the MIF -173 locus, and no deviation was observed from Hardy-Weinberg equilibrium. The final results were the same completely, whatever either tetra-primer ARMS or RFLP-PCR was used to check the MIF -173 single nucleotide polymorphism. Statistical analysis showed that the distributions of MIF -173 SNP alleles and genotype frequencies were significantly different between Chinese Han population and European Caucasian(P< 0.01), but no significant difference demonstrated to happen between Chinese and Japanese(P> 0.05).
CONCLUSIONTetra-primer ARMS is an accurate, rapid and economical method for SNP genotyping.There exists ethnic difference in the distribution of MIF -173 SNP alleles.
