An investigation for genetic polymorphisms of 10 STR loci on chromosome X of Chinese Hui nationality population in Ningxia region of China.
- Author:
Xue FENG
1
;
Ping XU
;
Ya TUO
;
Bing YU
;
Sheng-bin LI
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Child; China; Chromosomes, Human, X; genetics; Electrophoresis, Polyacrylamide Gel; Female; Gene Frequency; Genotype; Humans; Linkage Disequilibrium; Male; Microsatellite Repeats; genetics; Polymerase Chain Reaction; Polymorphism, Genetic; genetics
- From: Chinese Journal of Medical Genetics 2006;23(3):346-348
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the alleles and genotypes frequency of 10 short tandem repeat (STR) loci (DXS101, DXS6789, DXS6799, DXS6804, DXS7130, DXS7132, DXS7133, DXS7423, HPRTB, DXS8378) on X chromosome of Chinese Hui nationality population.
METHODSThe study of 10 STR loci was performed by using the techniques of PCR, polyacylamide gel electrophoresis and silver staining.
RESULTSAmong unrelated Hui individuals, the allele numbers of 10 STR loci DXS101, DXS6789, DXS6799, DXS6804, DXS7130, DXS7132, DXS7133, DXS7423, HPRTB, DXS8378 were 9, 8, 4, 6, 6, 6, 4, 4, 5 or 5 respectively; the numbers of genotypes were 17, 22, 7, 14, 14, 15, 6, 7, 12 or 8 respectively. The distribution of genotypes from these 10 STR systems fitted the Hardy-Weinberg equilibrium (P> 0.05). Polymorphisms information content of 10 STR loci, except for DXS7133 (0.48) and DXS7423 (0.48), ranged from 0.54 (DXS6799) to 0.80 (DXS6789); the power of discrimination were from 0.89 (DXS7133, DXS7423) to 0.99 (DXS6789, DXS7132, DXS101).
CONCLUSIONThe loci of 10 STR on chromosome X are appropriate for individual identification, paternity testing involving a female child and for study on related disease.
